The facility on Sunday transferred about 120 combined patients from its main Parnassus campus, as well as its Mt. Zion facility.
Ambulances took patients to the new Mission Bay location, which is along the San Francisco waterfront, near Pier 54.
The 878,000 square foot facility actually consists of three hospitals, including a children's hospital and cancer center.
The Mission Bay medical center will have the world's largest fleet of mobile robots that are programmed to transport items such as meals, medications, and lab specimens, UCSF officials said.